Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Cell Line Origin

Aorta

Components

Rabbit Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

General description

RbAOSMC from Cell Applications, Inc. were used to demonstrate that anti-restenosis drug probucol inhibition of vascular smooth cell proliferation by enhancing apoptosis and inducing heme oxygenase-1 (Deng, 2004). RbAOSMC were also used to evaluate performance of a novel pH/CO2 sensor system for measuring metabolic activity of cultured cells (Mohri, 2006); and to characterize the receptor pharmacology, antiplatelet activity, and vascular pharmacology of APD791, a novel 5-HT2A receptor antagonist (Adams, 2009).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Preparation Note

2nd passage, >500,000 cells in Rabbit Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO Can be cultured at least 6 doublings

Subculture Routine

Please refer to the RbAOSMC Culture Protocol.